Publications – Klassen Group

2023 Publications

Tomris, I.; Unione, L.; Nguyen, L.; Zaree, P.; Bouwman, K. M.; Liu, L.; Li, Z.; Fok, J. A.; Ríos Carrasco, M.; van der Woude, R.; Kimpel, A. L. M.; Linthorst, M. W.; Kilavuzoglu, S. E.; Verpalen, E. C. J. M.; Caniels, T. G.; Sanders, R. W.; Heesters, B. A.; Pieters, R. J.; Jiménez-Barbero, J.; Klassen, J. S.; Boons, G.-J.; de Vries, R. P. SARS-CoV-2 Spike N-Terminal Domain Engages 9-O-Acetylated Α2–8-Linked Sialic Acids. ACS Chem. Biol. 2023. https://doi.org/10.1021/acschembio.3c00066.
Schmidt, E. N.; Lamprinaki, D.; McCord, K. A.; Joe, M.; Sojitra, M.; Waldow, A.; Nguyen, J.; Monyror, J.; Kitova, E. N.; Mozaneh, F.; Guo, X. Y.; Jung, J.; Enterina, J. R.; Daskhan, G. C.; Han, L.; Krysler, A. R.; Cromwell, C. R.; Hubbard, B. P.; West, L. J.; Kulka, M.; Sipione, S.; Klassen, J. S.; Derda, R.; Lowary, T. L.; Mahal, L. K.; Riddell, M. R.; Macauley, M. S. Siglec-6 Mediates the Uptake of Extracellular Vesicles through a Noncanonical Glycolipid Binding Pocket. Nat. Commun. 2023, 14 (1), 2327. https://doi.org/10.1038/s41467-023-38030-6.

2022 Publications

Gheorghita, A. A.; Li, Y. E.; Kitova, E. N.; Bui, D. T.; Pfoh, R.; Low, K. E.; Whitfield, G. B.; Walvoort, M. T. C.; Zhang, Q.; Codée, J. D. C.; Klassen, J. S.; Howell, P. L. Structure of the AlgKX Modification and Secretion Complex Required for Alginate Production and Biofilm Attachment in Pseudomonas Aeruginosa. Nat. Commun. 2022, 13 (1), 7631. https://doi.org/10.1038/s41467-022-35131-6.
Han, L.; Nguyen, L.; Schmidt, E. N.; Esmaili, M.; Kitova, E. N.; Overduin, M.; Macauley, M. S.; Klassen, J. S. How Choice of Model Membrane Affects Protein–Glycosphingolipid Interactions: Insights from Native Mass Spectrometry. Anal. Chem. 2022. https://doi.org/10.1021/acs.analchem.2c03067.
Forrester, T. J. B.; Ovchinnikova, O. G.; Li, Z.; Kitova, E. N.; Nothof, J. T.; Koizumi, A.; Klassen, J. S.; Lowary, T. L.; Whitfield, C.; Kimber, M. S. The Retaining β-Kdo Glycosyltransferase WbbB Uses a Double-Displacement Mechanism with an Intermediate Adduct Rearrangement Step. Nat. Commun. 2022, 13 (1), 6277. https://doi.org/10.1038/s41467-022-33988-1.
Tomris, I.; Unione, L.; Nguyen, L.; Zaree, P.; Bouwman, K. M.; Liu, L.; Li, Z.; Fok, J. A.; Ríos Carrasco, M.; van der Woude, R.; Kimpel, A. L. M.; Linthorst, M. W.; Verpalen, E. C. J. M.; Caniels, T. G.; Sanders, R. W.; Heesters, B. A.; Pieters, R. J.; Jiménez-Barbero, J.; Klassen, J. S.; Boons, G.-J.; de Vries, R. P. The SARS-CoV-2 spike N-terminal domain engages 9-O-acetylated α2-8-linked sialic acids. bioRxiv 2022, 2022.09.14.507904. https://doi.org/10.1101/2022.09.14.507904.
Bui, D. T.; Kitova, E. N.; Mahal, L. K.; Klassen, J. S. Mass Spectrometry-Based Shotgun Glycomics for Discovery of Natural Ligands of Glycan-Binding Proteins. Curr. Opin. Struct. Biol. 2022, 77, 102448. https://doi.org/10.1016/j.sbi.2022.102448.
Pfoh, R.; Subramanian, A. S.; Huang, J.; Little, D. J.; Forman, A.; DiFrancesco, B. R.; Balouchestani-Asli, N.; Kitova, E. N.; Klassen, J. S.; Pomès, R.; Nitz, M.; Howell, P. L. The TPR Domain of PgaA Is a Multifunctional Scaffold That Binds PNAG and Modulates PgaB-Dependent Polymer Processing. PLOS Pathog. 2022, 18 (8), e1010750.
Bui, D. T.; Li, Z.; Kitov, P. I.; Han, L.; Kitova, E. N.; Fortier, M.; Fuselier, C.; Granger Joly de Boissel, P.; Chatenet, D.; Doucet, N.; Tompkins, S. M.; St-Pierre, Y.; Mahal, L. K.; Klassen, J. S. Quantifying Biomolecular Interactions Using Slow Mixing Mode (SLOMO) Nanoflow ESI-MS. ACS Cent. Sci. 2022, 8, 7, 963–974. https://doi.org/10.1021/acscentsci.2c00215.
Park, H.; Osman, E. A.; Cromwell, C. R.; St Laurent, C. D.; Liu, Y.; Kitova, E. N.; Klassen, J. S.; Hubbard, B. P.; Macauley, M. S.; Gibbs, J. M. CRISPR-Click Enables Dual-Gene Editing with Modular Synthetic SgRNAs. Bioconjug. Chem. 2022. https://doi.org/10.1021/acs.bioconjchem.2c00106.
Bui, D. T.; Jung, J.; Kitova, E. N.; Li, Z.; Willows, S. D.; Boddington, M. E.; Kitov, P. I.; Mason, A. L.; Capicciotti, C. J.; Mahal, L. K.; Macauley, M. S.; Klassen, J. S. Mass Spectrometry-Based Shotgun Glycomics Using Labeled Glycan Libraries. Anal. Chem. 2022. https://doi.org/10.1021/acs.analchem.1c04779.

2021 Publications

Guy, S.; L, H. J.; E, H. B.; F, M. C.; A, L. J.; M, J. N.; Saumya, W.; C, D. J.; Elisha, G.; Amr, E.-H.; Linh, N.; S, K. J.; M, S. C.; B, L. C.; A, M. D. Fucosylated Human Milk Oligosaccharide Foraging within the Species Bifidobacterium Pseudocatenulatum Is Driven by Glycosyl Hydrolase Content and Specificity. Appl. Environ. Microbiol. 2021, 0 (ja), AEM.01707-21. https://doi.org/10.1128/AEM.01707-21
Nguyen, L.; McCord, K. A.; Bui, D. T.; Bouwman, K. M.; Kitova, E. N.; Elaish, M.; Kumawat, D.; Daskhan, G. C.; Tomris, I.; Han, L.; Chopra, P.; Yang, T.-J.; Willows, S. D.; Mason, A. L.; Mahal, L. K.; Lowary, T. L.; West, L. J.; Hsu, S.-T. D.; Hobman, T.; Tompkins, S. M.; Boons, G.-J.; de Vries, R. P.; Macauley, M. S.; Klassen, J. S. Sialic Acid-Containing Glycolipids Mediate Binding and Viral Entry of SARS-CoV-2. Nat. Chem. Biol. 2021. https://doi.org/10.1038/s41589-021-00924-1.
Li, Z.; Kitov, P. I.; Kitova, E. N.; Bui, D. T.; Moremen, K. W.; Wakarchuk, W. W.; Mahal, L. K.; Macauley, M. S.; Klassen, J. S. Quantifying Carbohydrate-Active Enzyme Activity with Glycoprotein Substrates Using Electrospray Ionization Mass Spectrometry and Center-of-Mass Monitoring. Anal. Chem. 2021. https://doi.org/10.1021/acs.analchem.1c02089.
Park, H.; Osman, E.; Cromwell, C.; Laurent, C.; Liu, Y.; Kitova, E.; Klassen, J.; Hubbard, B.; Macauley, M.; Gibbs, J. CRISPR-Click Enables Multi-Gene Editing with Modular Synthetic SgRNAs; 2021. https://doi.org/10.33774/chemrxiv-2021-7pdz9.
Jung, J.; Enterina, J. R.; Bui, D. T.; Mozaneh, F.; Lin, P.-H.; Nitin; Kuo, C.-W.; Rodrigues, E.; Bhattacherjee, A.; Raeisimakiani, P.; Daskhan, G. C.; St. Laurent, C. D.; Khoo, K.-H.; Mahal, L. K.; Zandberg, W. F.; Huang, X.; Klassen, J. S.; Macauley, M. S. Carbohydrate Sulfation As a Mechanism for Fine-Tuning Siglec Ligands. ACS Chem. Biol. 2021.
Báez Bolivar EG, Bui DT, Kitova EN, Han L, Zheng RB, Luber EJ, Sayed SY, Mahal LK, Klassen JS. Submicron Emitters Enable Reliable Quantification of Weak Protein-Glycan Interactions by ESI-MS. Anal Chem. 2021 Feb 25. doi: 10.1021/acs.analchem.0c05003.
Paraskevopoulou, V.; Schimpl, M.; Overman, R. C.; Stolnik, S.; Chen, Y.; Nguyen, L.; Winkler, G. S.; Gellert, P.; Klassen, J. S.; Falcone, F. H. Structural and Binding Characterization of the LacdiNAc-Specific Adhesin (LabA; HopD) Exodomain from Helicobacter Pylori. Curr. Res. Struct. Biol. 2021, 3, 19–29.

2020 Publications

Han L, Xue X, Roy R, Kitova EN, Zheng RB, St-Pierre Y, Lowary TL, Klassen JS.” Neoglycolipids as Glycosphingolipid Surrogates for Protein Binding Studies Using Nanodiscs and Native Mass Spectrometry“. Anal Chem. 2020. doi: 10.1021/acs.analchem.0c03344.
Park H, Jung J, Rodrigues E, Kitova EN, Macauley MS, Klassen JS. Mass Spectrometry-based Shotgun Glycomics for Discovery of Natural Ligands of Glycan-binding Proteins. Anal Chem. 2020 Sep 16. doi: 10.1021/acs.analchem.0c02931. Epub ahead of print. PMID: 32936606.
Li Z, Kitov PI, Kitova EN, Mozenah F, Rodrigues E, Chapla DG, Moremen KW, Macauley MS, Klassen JS. CUPRA-ZYME: An Assay for Measuring Carbohydrate-Active Enzyme Activities, Pathways, and Substrate Specificities. Anal Chem. 2020 Feb 18;92(4):3228-3236. doi: 10.1021/acs.analchem.9b05007. Epub 2020 Feb 7. PMID: 31961140.
Han L, Kitov PI, Li J, Kitova EN, Klassen JS. Probing Heteromultivalent Protein-Glycosphingolipid Interactions using Native Mass Spectrometry and Nanodiscs. Anal Chem. 2020 Mar 3;92(5):3923-3931. doi: 10.1021/acs.analchem.9b05419. Epub 2020 Feb 12. PMID: 31944669.
Rodrigues E, Jung J, Park H, Loo C, Soukhtehzari S, Kitova EN, Mozaneh F, Daskhan G, Schmidt EN, Aghanya V, Sarkar S, Streith L, St Laurent CD, Nguyen L, Julien JP, West LJ, Williams KC, Klassen JS, Macauley MS. A versatile soluble siglec scaffold for sensitive and quantitative detection of glycan ligands. Nat Commun. 2020 Oct 9;11(1):5091. doi: 10.1038/s41467-020-18907-6. PMID: 33037195; PMCID: PMC7547722.
Bamford NC, Le Mauff F, Van Loon JC, Ostapska H, Snarr BD, Zhang Y, Kitova EN, Klassen JS, Codée JDC, Sheppard DC, Howell PL. Structural and biochemical characterization of the exopolysaccharide deacetylase Agd3 required for Aspergillus fumigatus biofilm formation. Nat Commun. 2020 May 15;11(1):2450. doi: 10.1038/s41467-020-16144-5. PMID: 32415073; PMCID: PMC7229062.
Eddenden A, Kitova EN, Klassen JS, Nitz M. An Inactive Dispersin B Probe for Monitoring PNAG Production in Biofilm Formation. ACS Chem Biol. 2020 May 15;15(5):1204-1211. doi: 10.1021/acschembio.9b00907. Epub 2020 Jan 21. PMID: 31917539.

2019 Publications

Kitova EN, Han L, Vinals DF, Kitov PI, Derda R, Klassen JS. Influence of labeling on the glycan affinities and specificities of glycan-binding proteins. A case study involving a C-terminal fragment of human galectin-3. Glycobiology. 2019 Dec 12;30(1):49-57. doi: 10.1093/glycob/cwz076. PMID: 31529034.
Kitov, PI, Kitova, EN, Han, L., Li, Z., Jung, J., Rodrigues, E., Hunter, CD, Cairo, CW, Macauley, MS, Klassen, JS “A quantitative, high-throughput method identifies protein–glycan interactions via mass spectrometry” Communications biology 2019, 2, 1, 268.
Kitov, PI, Han, L., Kitova, EN, Klassen, JS “Sliding Window Adduct Removal Method (SWARM) for Enhanced Electrospray Ionization Mass Spectrometry Binding Data” Journal of The American Society for Mass Spectrometry 2019, 30, 8,1-9.
Wang Y, Park H, Lin H, Kitova EN, Klassen JS “Multipronged ESI-MS Approach for Studying Glycan-Binding Protein Interactions with Glycoproteins” Analytical Chemistry 2019, 91, 3, 2140-2147
Wei Y, Xiong ZJ, Li J, Zou C, Cairo CW, Klassen JS, Privé GG. Crystal structures of human lysosomal EPDR1 reveal homology with the superfamily of bacterial lipoprotein transporters. Commun Biol. 2019 Feb 5;2:52. doi: 10.1038/s42003-018-0262-9. PMID: 30729188; PMCID: PMC6363788.
Ryan P, Forrester TJB, Wroblewski C, Kenney TMG, Kitova EN, Klassen JS, Kimber MS. The small RbcS-like domains of the β-carboxysome structural protein CcmM bind RubisCO at a site distinct from that binding the RbcS subunit. J Biol Chem. 2019 Feb 22;294(8):2593-2603. doi: 10.1074/jbc.RA118.006330. Epub 2018 Dec 27. PMID: 30591587; PMCID: PMC6393606.

2018 Publications

Xue X, Zheng RB, Koizumi A, Han L, Klassen JS, Lowary TL “Synthetic Polyprenol-Pyrophosphate Linked Oligosaccharides Are Efficient Substrates for Mycobacterial Galactan Biosynthetic Enzymes” Org. Biomol. Chem. 2018, 16, 1939-1957 (selected as HOT article)
Han L, Zheng R, Richards MR, Tan M, Kitova EN, Jiang X, Klassen JS “Quantifying the Binding Stoichiometry and Affinity of Histo-Blood Group Antigen Oligosaccharides for Human Noroviruses” Glycobiology 2018, 28, 488–498.
Carlson M; Young J, Zhao Z, Fabre L, Jun D, Li J (Jun), Li, J (Jianing), Dhupar H, Mills A, Beatty JT, Klassen JS, Rouiller I, Duong F “The peptidisc, a simplified approach for stabilizing membrane proteins in detergent-free solution” eLife, 2018
Jun Li, Ling Han, Jianing Li, Elena N. Kitova, Zi Jian Xiong, Gilbert G. Privé, John S. Klassen “Detecting Protein-Glycolipid Interactions using CaR-ESI-MS and Model Membranes. Comparison of Pre- and Passively-loaded Picodiscs” J. Am. Soc. Mass. Spectrom. 2018, 29,7,1493-1504.
Carmanah D. Hunter, Neha Khanna, Michele R. Richards, Reza Rezaei Darestani, Chunxia Zou, John S. Klassen, and Christopher W. Cairo “Human Neuraminidase Isoenzymes Show Variable Activities for 9-O-Acetyl-sialoside Substrates” Chemical Biology 2018 13 (4), 922-932
Ying Chou, Elena N. Kitova, Maju Joe, Richard Brunton, Todd L. Lowary, John S. Klassen and Ratmir Derda “Genetically-encoded fragment-based discovery (GE-FBD) of glycopeptide ligands with differential selectivity for antibodies related to mycobacterial infections” Org. Biomol. Chem. 2018 16: 223-227
Amr El-Hawiet, Yajie Chen, Km Shams-Ud-Doha, Elena N. Kitova, Pavel I. Kitov, Lars Bode, Naim Hage, Franco H. Falcone and John S. Klassen “Screening natural libraries of human milk oligosaccharides against lectins using CaR-ESI-MS” Analyst 2018 143, 536-548
Xia M, Huang P, Sun C, Han L, Vago FS, Li K, Zhong W, Jiang W, Klassen JS, Jiang X, Tan M. Bioengineered Norovirus S₆₀ Nanoparticles as a Multifunctional Vaccine Platform. ACS Nano. 2018 Nov 27;12(11):10665-10682. doi: 10.1021/acsnano.8b02776. Epub 2018 Sep 25. PMID: 30234973; PMCID: PMC6261714.

2017 Publications

Amr El-Hawiet, Yajie Chen, Km Shams-Ud-Doha, Elena N Kitova, Yves St-Pierre, and John S. Klassen“High Throughput, Label- and Immobilization-Free Screening of Human Milk Oligosaccharides Against Lectins” Analytical chemistry 2017 89 (17): 8713–8722
Ling Han, Luis Morales, Michele R Richards, Elena N Kitova, Simonetta Sipione, and John S. Klassen“Investigating the Influence of Membrane Composition on Protein-Glycolipid Binding Using Nanodiscs and Proxy Ligand ESI-MS” Analytical chemistry 2017 89 (17):9330–9338
Jun Li, Michele R. Richards, Elena N. Kitova, and John S. Klassen “Delivering Transmembrane Peptide Complexes to the Gas Phase Using Nanodiscs and Electrospray Ionization” J Am Soc Mass Spectrom. 2017 28:2054-2065
Km Shams-Ud-Doha, Elena N. Kitova, Pavel I. Kitov, Yves St-Pierre, and John S. Klassen “Human Milk Oligosaccharide Specificities of Human Galectins. Comparison of Electrospray Ionization Mass Spectrometry and Glycan Microarray Screening Results” Analytical chemistry 2017 89(9):4914-4921
Wei Zhang, Alexander W Lohman, Yevgeniya Zhuravlova, Xiaocen Lu, Matthew D Wiens, Hiofan Hoi, Sine Yaganoglu, Manuel A Mohr, Elena N Kitova, John S Klassen, Periklis Pantazis, Roger J Thompson& Robert E Campbell “Optogenetic control with a photocleavable protein, PhoCl” Nature Methods 2017 14:391-394
Elena N. Kitova, Yuyu Yao, and John S. Klassen “Stabilizing protein-ligand complexes in ESI–MS using solution additives: Comparing the effects of amino acids and imidazole” International Journal of Mass Spectrometry 2017 240:2-8

2016 Publications

Ling Han, Elena N. Kitova and John S. Klassen”Detecting Protein-Glycolipid Interactions using Glycomicelles and CaR-ESI-MS” J Am Soc Mass Spectrom. 2016, 27:1878
Jun Li, Michele R. Richards, Dhanashri Bagal, Iain D. G. Campuzano, Elena N. Kitova, Zi Jian Xiong, Gilbert G. Privé, and John S. Klassen “Characterizing the Size and Composition of Saposin A Lipoprotein Picodiscs” Analytical chemistry 2016, 88 (19): 9524-9531
Ling Han (contributed equally), Km Shams-Ud-Doha (contributed equally), Elena N Kitova, John S Klassen”Screening Oligosaccharide Libraries against Lectins Using the Proxy Protein ESI-MS assay” Analytical chemistry 2016, 88(16):8224-8231
Lan Liu, Iwona Siuda, Michele Richards, Justin Renaud, Elena Kitova, Paul Mayer, Peter Tieleman, Todd Lowary andJohn S. Klassen”Structure and Stability of Carbohydrate-Lipid Interactions. Methylmannose Polysaccharide-Fatty Acid Complexes” ChemBioChem. 2016, 17(16):1571-1578
Jun Li, Xuxin Fan, Elena N Kitova, Chunxia Zou, Christopher W. Cario, Luiz Eugenio, Kenneth KS Ng, Zi Jiang Xiong, Gilber G. Prive and John S. Klassen “Screening Glycolipids Against Proteins in vitro using Picodiscs and Catch-and-Release Electrospray Ionization Mass Spectrometry” Analytical chemistry 2016, 88(9):4742-4750
Reza R. Darestani, Philip Winter, Elena N. Kitova, Jack A. Tuszynski and John S. Klassen ” Screening Anti-Cancer Drugs against Tubulin using Catch-and-Release Electrospray Ionization Mass Spectrometry” J Am Soc Mass Spectrom. 2016, 27(5):876-885
Yuyu Yao, Michele R. Richards, Elena N. Kitova and John S. Klassen ” Influence of Sulfolane on ESI-MS Measurements of Protein–Ligand Affinities. J Am Soc Mass Spectrom. 2016, 27(3) : 498-506
Jingjing Zhang, Elena N. Kitova, Jun Li, Luiz Eugenio, Kenneth KS Ng and John S. Klassen ” Localizing Carbohydrate Binding Sites in Proteins Using Hydrogen/Deuterium Exchange Mass Spectrometry” J Am Soc Mass Spectrom. 2016, 27(1):83-90
Katrina F. Tjhung, Pavel I. Kitov, Simon Ng, Elena N. Kitova, Lu Deng, John S. Klassen, and Ratmir Derda “Silent Encoding of Chemical Post-Translational Modifications in Phage-Displayed Libraries” J. Am. Chem. Soc. 2016, 138, 32-35

2015 Publications

Amr El-Hawiet, Elena N. Kitova, and John S. Klassen “Recognition of human milk oligosaccharides by bacterial exotoxins” Glycobiology. 2015, 25(8):845-54
Simon Ng, Edith Lin, Pavel I. Kitov, Katrina F. Tjhung, Oksana O. Gerlits, Lu Deng, BrianKasper, Amika Sood, Beth M. Paschal, Ping Zhang, Chang-Chun Ling, John S. Klassen,Christopher J. Noren, Lara K. Mahal, Robert J. Woods, Leighton Coates, and Ratmir Derda “Genetically-encoded Fragment-based Discovery ofGlycopeptide Ligands for Carbohydrate-binding Proteins” J. Am. Chem. Soc., 2015, 137, 5248-5251
Ling Han, Elena N. Kitova, Jun Li, Sanaz Nikjah, Hong Lin, Benjamin Pluvinage, Alisdair B. Boraston, and John S. Klassen “Protein-Glycolipid Interactions Studied in vitro using ESI-MS and Nanodiscs. Insights into the Mechanisms and Energetics of Binding” Anal Chem. 2015, 87, 4888-4896
Aneika C. Leney, Reza Rezaei Darestani, Jun Li, Sanaz Nikjah, Elena N. Kitova, Chunxia Zou, Christopher W. Cairo, Zi Jian Xiong, Gilbert G. Privé , and John S. Klassen “Picodiscs for Facile Protein-Glycolipid Interaction Analysis” Anal Chem. 2015, 87, 4402-4408
Carlos A. Sanhueza, Jonathan Cartmell, Amr El-Hawiet, Adam Szpacenko, Elena N. Kitova, Rambod Daneshfar, John Klassen, Kenneth K.-S. Ng, Luiz Eugenio, Pavel I. Kitov, and David R. Bundle “Evaluation of a Focused Virtual Library of Heterobifunctional Ligands for Clostridium difficile Toxins” Organic & Biomolecular Chemistry. 2015, 13(1), 283-298
Ling Han, Elena N. Kitova, Ming Tan, Xi Jiang, Benjamin Pluvinage, Alisdair B. Boraston and John S. Klassen “Affinities of Human Histo-Blood Group Antigens for Norovirus Capsid Protein Complexes” Glycobiology. 2015, 25(2), 170-180
Yuyu Yao, Km Shams-Ud-Doha, Rambod Daneshfar, Elena N. Kitova and John S. Klassen “Quantifying Protein-Carbohydrate Interactions Using Liquid Sample Desorption Electrospray Ionization Mass Spectrometry” J Am Soc Mass Spectrom. 2015, 26(1), 98-106
Ming Tan, Chao Wei, Pengwei Huang, Qiang Fan, Christina Quigley, Ming Xia, Hao Fang, Xufu Zhang, Weiming Zhong, John S. Klassen & Xi Jiang “Tulane virus recognizes sialic acids as cellular receptors” Sci. Rep. 2015, 5, Ariticle number: 11784
Wenwen Xu, Yuyu Yao, John S. Klassena and Michael J. Serpe “Magnetic field assisted programming of particle shapes and patterns” Soft Matter 2015, 11, 7151-7158

2014 Publications

Perrin Baker, Tyler Ricer, Patrick J. Moynihan, Elena N. Kitova, Marthe T. C. Walvoort, Dustin J. Little, John C. Whitney, Karen Dawson, Joel T. Weadge, Howard Robinson, Dennis E. Ohman, Jeroen D. C. Codée, John S. Klassen, Anthony J. Clarke, P. Lynne Howell “P. aeruginosa SGNH Hydrolase-Like Proteins AlgJ and AlgX Have Similar Topology but Separate and Distinct Roles in Alginate Acetylation” PLoS Pathog. 2014, 10(8), e1004334
Elena N. Kitova, Amr El-Hawiet, and John S. Klassen “Screening Carbohydrate Libraries for Protein Interactions using the Direct ESI-MS Assay. Applications to Libraries of Unknown Concentration” Journal of The American Society for Mass Spectrometry. 2014, 25(11), 1908-1916
Ling Han, Ming Tan, Ming Xia, Elena N. Kitova, Xi Jiang, and John S. Klassen “Gangliosides are Ligands for Human Noroviruses” J. Am. Chem. Soc., 2014, 136(36), 12631-12637
Aneika C Leney, Xuxin Fan, Elena N Kitova, John S. Klassen “Nanodiscs and Electrospray Ionization Mass Spectrometry. A Novel Tool for Screening Glycolipids Against Proteins” Anal. Chem., 2014, 86(11), 5271-5277
Lan Liu, Nobar Jalili, Alyson Baergen, Simon Ng, Justin Bailey, Ratmir Derda, John S. Klassen “Fluorine Bonding Enhances the Energetics of Protein-Lipid Binding in the Gas Phase” Journal of The American Society for Mass Spectrometry. 2014, 25(5), 751-757
Lan Liu, Alyson Baergen, Klaus Michelsen, Elena N. Kitova, Paul D. Schnier, John S. Klassen “Energetics of Intermolecular Hydrogen Bonds in a Hydrophobic Protein Cavity” Journal of The American Society for Mass Spectrometry. 2014, 25(5), 742-750
Wolfram F, Kitova EN, Robinson H, Walvoort M, Codée JD, Klassen JS, Howell PL “Catalytic mechanism and mode of action of the periplasmic alginate epimerase AlgG” J Biol Chem. 2014, 289(9),6006-6019
Murase T, Eugenio L, Schorr M, Hussack G, Tanha J, Kitova EN, Klassen JS, Ng KK “Structural Basis for Antibody Recognition in the Receptor-Binding Domains of Toxins A and B from Clostridium difficile” J Biol Chem. 2014, 289(4), 2331-2343
Jafari MR, Deng L, Kitov PI, Ng S, Matochko WL, Tjhung KF, Zeberoff A, Elias A, Klassen JS, Derda R “Discovery of Light-Responsive Ligands through Screening of a Light-Responsive Genetically Encoded Library” ACS Chem Biol. 2014, 9(2), 443-450
Hong Lin, Elena N. Kitova, John S. Klassen “Measuring Positive Cooperativity Using the Direct ESI-MS Assay. Cholera Toxin B Subunit Homopentamer Binding to GM1 Pentasaccharide” Journal of The American Society for Mass Spectrometry. 2014, 25(1), 104-110
Ling Han, Elena N. Kitova, Ming Tan, Xi Jiang, John S. Klassen “Identifying Carbohydrate Ligands of a Norovirus P Particle using a Catch and Release Electrospray Ionization Mass Spectrometry Assay” Journal of The American Society for Mass Spectrometry. 2014, 25(1), 111-119
Denis Arutyunov, Upasana Singh, Amr El-Hawiet, Henrique dos Santos Seckler, Sanaz Nikjah, Maju Joe, Yu Bai, Todd L Lowary, John S Klassen, Stephane Evoy, and Christine M Szymanski “Mycobacteriophage cell binding proteins for the capture of mycobacteria” Bacteriophage 2014, e960346, 1-9

2013 Publications

Hong Lin, Elena N. Kitova , and John S. Klassen “Quantifying Protein–Ligand Interactions by Direct Electrospray Ionization-MS Analysis: Evidence of Nonuniform Response Factors Induced by High Molecular Weight Molecules and Complexes” Anal. Chem., 2013, 85(19), 8919-8922
Amr El-Hawiet , Elena N. Kitova , and John S. Klassen “Quantifying Protein Interactions with Isomeric Carbohydrate Ligands Using a Catch and Release Electrospray Ionization-Mass Spectrometry Assay” Anal. Chem., 2013, 85(16), 7637-7644
Yixuan Zhang, Lu Deng, Elena N. Kitova, John S. Klassen “Dissociation of Multisubunit Protein–Ligand Complexes in the Gas Phase. Evidence for Ligand Migration” Journal of The American Society for Mass Spectrometry. 2013, 24, 1573-1583
Lu Deng, Elena N. Kitova, John S. Klassen. “Mapping Protein–Ligand Interactions in the Gas Phase Using a Functional Group Replacement Strategy. Comparison of CID and BIRD Activation Methods” Journal of The American Society for Mass Spectrometry. 2013, 24, 988-996
Fumie X. Sunahori, Guochun Yang, Elena N. Kitova, John S. Klassen and Yunjie Xu. “Chirality recognition of the protonated serine dimer and octamer by infrared multiphoton dissociation spectroscopy” Phys. Chem. Chem. Phys., 2013, 15, 1873-1886
Lu Deng, Elena N. Kitova, and John S. Klassen. “Dissociation Kinetics of the Streptavidin-Biotin Interaction Measured using Direct Electrospray Ionization Mass Spectrometry Analysis” Journal of The American Society for Mass Spectrometry. 2013, 24(1), 49-56
Ling Han, Pavel I. Kitov, Elena N. Kitova, Ming Tan, Leyi Wang, Ming Xia, Xi Jiang, and John S. Klassen. “Affinities of Recombinant Norovirus P Dimers for Human Blood Group Antigens” Glycobiology. 2013, 23(3), 276-285
Elena N. Kitova, Igor Sinelnikov, Lu Deng and John S. Klassen “Thermal Dissociation of Streptavidin Homotetramer in the Gas Phase. Subunit Loss versus Backbone Fragmentation” Int. J. Mass spectrom. , 2013, 345-347, 97-103.

2012 Publications

Deng, Lu; Broom, Aron; Kitova, Elena; Richards, Michele; Zheng, Ruixiang; Shoemaker, Glen; Meiering, Elizabeth; Klassen, John “Kinetic Stability of the Streptavidin-Biotin Interaction Enhanced in the Gas Phase ” J. Am. Chem. Soc., 2012, 134(40), 16586-16596
Yixuan Zhang, Lan Liu, Rambod Daneshfar, Elena N. Kitova, Caishun Li, Feng Jia, Christopher W. Cairo and John S. Klassen “Protein-Glycosphingolipid Interactions Revealed using Catch-and-Release Mass Spectrometry” Anal. Chem., 2012, 84(18), 7618-7621
Hong Lin, Elena N. Kitova, Margaret A. Johnson, Luiz Eugenio, Kenneth K.S. Ng, and John S. Klassen “Electrospray Ionization-Induced Protein Unfolding” J. Am. Soc. Mass Spectrom, 2012, 23(12), 2122-2131
Lan Liu, Yu Bai, Nian Sun, Li Xia, Todd L. Lowary and John S. Klassen “Carbohydrate–Lipid Interactions. Affinities of Methylmannose Polysaccharides for Lipids in Aqueous Solution” Chemistry – A European Journal, 2012, 18(38), 12059-12067
El-Hawiet, A., Kitova, E.N.,and Klassen, J.S. “Quantifying Carbohydrate-Protein Interactions by Electrospray Ionization Mass Spectrometry Analysis” Biochemistry, 2012, 51(21), 4244-4253
El-Hawiet, A., Kitova, E.N., Arutyunov, D., Szymanski, C.M., and Klassen, J.S. “Quantifying ligand binding to large protein complexes using electrospray ionization mass spectrometry” Anal.Chem., 2012, 84(9), 3867–3870
Lan Liu, Klaus Michelsen, Elena N. Kitova, Paul D. Schnier, Alex Brown, and John S. Klassen “Deuterium Kinetic Isotope Effects on the Dissociation of a Protein–Fatty Acid Complex in the Gas Phase” J. Am. Chem. Soc. 2012, 134, 5931–5937
Liu, Lan; Michelsen, Klaus; Kitova, Elena; Schnier, Paul; Klassen, John “Energetics of Lipid Binding in a Hydrophobic Protein Cavity” J. Am. Chem. Soc. 2012, 134, 3054-3060
El-haweit, Amr; Shoemaker, Glen; Daneshfar, Rambod; Kitova, Elena; Klassen, John “Applications of a Catch and Release Electrospray Ionization Mass Spectrometry Assay for Carbohydrate Library Screening” Anal.Chem., 2012, 84, 50-58
Elena N. Kitova, Amr El-Hawiet, Paul D. Schnier and John S. Klassen “Reliable Determinations of Protein-Ligand Interactions by Direct ESI-MS Measurements. Are We There Yet?” J. Am. Soc. Mass Spectrom 2012, 23, 431-441

2011 Publications

Mahendra S. Sandbhor, Naoto Soya, Amgad Albohy, R. Blake Zheng, Jonathan Cartmell, David R. Bundle, John S. Klassen, and Christopher W. Cairo “Substrate Recognition of the Membrane-Associated Sialidase NEU3 Requires a Hydrophobic Aglycone” Biochemistry 2011, 50, 6753-6762
Ping Zhang, Nahid Razi, Luiz Eugenio, Messele Fentabil, Elena N. Kitova, John S. Klassen, David R. Bundle, Kenneth K.-S. Ng and Chang-Chun Ling “Ligands for C. difficile toxins:Unexpected structure of a pentasaccharide produced by enzymatic fucosylation and corrected annotation in a popular screening library” Chem. Comm. 2011, 47, 12397-12399
Isabelle Hug, Blake Zheng, Bela Reiz, Randy Whittal, Messele A. Fentabil, John S. Klassen, and Mario F. Feldman “Exploiting bacterial glycosylation machineries for the synthesis of a Lewis antigen-containing glycoprotein ” J. Biol. Chem. 2011, 286, 37887-37894
Messele A. Fentabil, Rambod Daneshfar, Elena N. Kitova and John S. Klassen “Blackbody Infrared Radiative Dissociation of Protonated Oligosaccharides” J. Am. Soc. Mass Spectrom, 2011, 22, 2171-2178
Kitova EN, Soya N, Klassen JS. “Identifying Specific Small Molecule Interactions Using Electrospray Ionization Mass Spectrometry” Anal. Chem. 2011, 83, 5160–5167
Amr El-Hawiet, Elena N. Kitova, Pavel Kitov, Luiz Eugenio, Kenneth K.S. Ng, George L. Mulvey, Tanis C. Dingle, Adam Szpacenko, Glen D. Armstrong and John S. Klassen “Binding of Clostridium difficile Toxins to Human Milk Oligosaccharides” Glycobiology, 2011, 21,1217-1227
Soya N, Fang Y, Palcic MM, Klassen JS. “Trapping and Characterization of Covalent Intermediates of Mutant Retaining Glycosyltransferases” Glycobiology , 2011, 21, 547-552
Liu L, Kitova EN, Klassen JS. “Quantifying Protein-Fatty Acid Interactions using Electrospray Ionization Mass Spectrometry ” J Am Soc Mass Spectrom, 2011, 22, 310-318

2010 Publications

Liu L, Michelsen K, Kitova EN, Schnier PD, Klassen JS. “Evidence that Water Can Reduce the Kinetic Stability of Protein-Hydrophobic Ligand Interaction” J. Am. Chem. Soc. , 2010, 132, 17658-17660
Hakansson K and Klassen JS. “Ion Activation Methods for Tandem Mass Spectrometry”Electrospray and MALDI Mass Spectrometry: Fundamentals, Instrumentation, Practicalities, and Biological Applications, Second Edition 2010, Chapter 16 (Editor: Richard B. Cole), WILEY, 571-630.
El-Hawiet A, Kitova EN, Liu L, Klassen JS. “Quantifying Labile Protein-Ligand Interactions using Electrospray Ionization Mass Spectrometry” J Am Soc Mass Spectrom 2010, 21, 1893-1899
Deng L, Sun N, Kitova EN, Klassen JS. “Direct Quantification of Protein-Metal Ion Affinities by Electrospray Ionization Mass Spectrometry” Anal. Chem. 2010, 82, 2170-2174
Sun N, Soya N, Kitova EN, Klassen JS. “Nonspecific Interactions between Proteins and Charged Biomolecules in Electrospray Ionization Mass Spectrometry” J Am Soc Mass Spectrom 2010, 21, 472-481

2009 Publications

Liu L, Bagal D, Kitova EN, Schnier PD, Klassen JS. “Hydrophobic Protein – Ligand Interactions Preserved in the Gas Phase” J. Am. Chem. Soc 2009, 131, 15980-15981
Soya N, Shoemaker GK, Palcic MM, Klassen JS. “Comparative Study of Substrate and Product Binding to the Human ABO(H) Blood Group Glycosyltransferases” Glycobiology 2009, 19, 1224-1234
Bagal D, Kitova EN, Liu L, El-Hawiet A, Schnier PD, Klassen JS. “Gas Phase Stabilization of Noncovalent Protein Complexes Formed by Electrospray Ionization” Anal. Chem. 2009, 81, 7801-7806.
Kitova EN, Mulvey GL, Dingle T, Sinelnikov I, Wee S, Griener TP, Armstrong GD, Klassen JS. “Assembly and stability of the shiga toxins investigated by electrospray ionization mass spectrometry” Biochemistry 2009, 48, 5365-5374.
Humphries RM, Donnenbberg MS, Strecher J, Kitova EN, Klassen JS, Cui L, Griener TP, Mulvey GL, Armstrong GD. “From alpha to beta: identification of amino acids requied for the N-acetyllactosamine specific lectin-like activity of bundlin” Mol Microbiol. 2009, 72, 859-68.
Sun N, Sun J, Kitova EN, Klassen JS. “Identifying Nonspecific Ligand Binding in Electrospray Ionization Mass Spectrometry Using the Reporter molecule Method” J Am Soc Mass Spectrom. 2009, 20, 1242-1250.

2008 Publications

Shoemaker GK, Soya N, Palcic MM, Klassen JS. “Temperature-dependent cooperativity in donor-accepator substrate bingding to the human blood group glycosltransferases” Glycobiology 2008, 18, 587-92.
Dingle T, Wee S, Mulvey GL, Greco A, Kitova EN, Sun J, Lin S, Klassen JS, Palcic MM, Ng KK, Armstrong GD “Functional properties of the carboxy-terminal host cell-binding domais of the two toxins, TcdA and TcdB, expressed by Clostridium difficile” Glycobiology 2008, 18,698-706.
P.I. Kitov, T. Lipinski, E. Paszkiewicz, D. Solomon, J.M. Sadowska, G.A. Grant, G.L. Mulvey, E.N. Kitova, J.S. Klassen, G.D. Armstrong and D.R. Bundle “An entropically efficient supramolecular inhibition strategy for Shiga toxins” Angew. Chemie Int. Ed. 2008, 47, 672-676.
E.N. Kitova, M. Seo, P.-N. Roy and J.S. Klassen “Elucidating the Intermolecular Interactions within a Desolvated Protein-Ligand Complex. An Experimental and Computational Study” J. Am. Chem. Soc. 2008, 130, 1214-1226.
R.M. Hyland, J.Sun, T.P. Griener, G.L. Mulvey, J.S. Klassen, M.S. Donnenberg, G.D. Armstrong “The bundlin pilin protein of enteropathogenic Escherichia coli is an N-acetyllactosamine specific lectin” Cell. Microbiol. 2008, 10, 177-187.

2007 Publications

A. Faridmoayer, M.A. Fentabil, D.C. Mills, J.S. Klassen and M.F. Feldman “Functional Characterization of Bacterial Oligosaccharyltransferases Involved in O-linked Protein Glycosylation” J. Bacteriol. 2007, 189, 8088-8098.
J. Sun, E.N. Kitova, N. Sun and J.S. Klassen “Method for Identifying Nonspecific Protein-Protein Interactions in Nanoelectrospray Ionization Mass Spectrometry” Anal. Chem. 2007, 79, 8301-8311.
E.N. Kitova, P.I. Kitov, E. Paszkiewicz, J. Kim, G.L. Mulvey, G.D. Armstrong, D.R. Bundle and J.S. Klassen “Affinities of Shiga Toxins 1 and 2 for Univalent and Oligovalent Pk Trisaccharide Analogs Measured by Electrospray Ionization Mass Spectrometry” Glycobiology 2007, 17, 1127-1137.
C. Rademacher, G.K. Shoemaker, H.-S. Kim, R.B. Zheng, H. Taha, C. Liu, R.C. Nacario, D.C. Schriemer, J.S. Klassen, T. Peters and T.L. Lowary “Ligand specificity of CS-35, a monoclonal antibody that recognizes mycobacterial lipoarabinomannan. A model system for oligofuranoside-protein recognition” J. Am. Chem. Soc. 2007, 129, 10489-10502.
G.K. Shoemaker, E.N. Kitova, M.M. Palcic and J.S. Klassen “Equivalency of Binding Sites in Protein-Ligand Complexes Revealed by Time-Resolved Tandem Mass Spectrometry “ J. Am. Chem. Soc. 2007, 129, 8674-8675.
I. Sinelnikov, E.N. Kitova, G.D. Armstrong and J.S. Klassen “Effects of Single Amino Acid Substitution on the Dissociation of Multiply Charged Multiprotein Complexes in the Gas Phase” J. Am. Soc. Mass Spectrom. 2007, 18, 688-692.
I. Sinelnikov, E.N. Kitova and J.S. Klassen “Influence of Coulombic Repulsion on Dissociation Pathways and Energetics of Multiprotein Complexes in the Gas Phase” J. Am. Soc. Mass Spectrom. 2007, 18, 617-631.
J. Sun, E.N. Kitova and J.S. Klassen “Method for Stabilizing Protein-Ligand Complexes in Nanoelectrospray Ionization Mass Spectrometry” Anal. Chem. 2007, 79, 417-425.

2006 Publications

G.K. Shoemaker, E.N. Kitova, and J.S. Klassen “Protein – Carbohydrate Affinities: An Example of a Direct Method to Determine Equilibrium Constants” The Encyclopedia of Mass Spectrometry 2006, Vol. 6 (Editors: M. L. Gross and R. M. Caprioli), Elsevier, Amsterdam , 810-822.
J. Sun, E.N. Kitova, W. Wang and J.S. Klassen “Method for Distinguishing Specific and Nonspecific Protein-Ligand Complexes in Nanoelectrospray Ionization Mass Spectrometry” Anal. Chem. 2006, 78, 3010-3018.
R. Daneshfar and J.S. Klassen “Thermal Decomposition of Multiply Charged T-Rich Oligonucleotide Anions in the Gas Phase. Influence of Internal Solvation on the Arrhenius Activation Parameters for Neutral Base Loss” J. Am. Soc. Mass Spectrom. 2006, 17, 1229-1238.

2005 Publications

E.N. Kitova, R. Daneshfar, P. Marcato, G. Mulvey, G.D. Armstrong and J.S. Klassen “Stability of the Homopentameric B Subunits of Shiga Toxins 1 and 2 in Solution and the Gas Phase as Revealed by NanoES-FT-ICR/MS” J. Am. Soc. Mass Spectrom. 2005, 16, 1957-1968.
W. Wang, E.N. Kitova, J. Sun and J.S. Klassen “Blackbody Infrared Radiative Dissociation Of Nonspecific Protein-Carbohydrate Complexes Produced by Nanoelectrospray Ionization. The Nature of the Noncovalent Interactions” J. Am. Soc. Mass Spectrom. 2005, 16, 1583-1594.
W. Wang, E.N. Kitova and J.S. Klassen “Nonspecific Protein-Carbohydrate Complexes Produced by Nanoelectrospray Ionization. Factors Influencing their Formation and Stability” Anal. Chem. 2005, 77, 3060-3071.

2004 Publications

E.N. Kitova, D.R. Bundle and J.S. Klassen “Partitioning of Solvent Effects and Intrinsic Interactions in Biological Recognition” Angew. Chemie Int. Ed. 2004, 43, 4183-4186.
R. Daneshfar, E.N. Kitova and J.S. Klassen “Determination of Protein-Ligand Association Thermochemistry Using Variable-Temperature Nanoelectrospray Mass Spectrometry” J. Am. Chem. Soc. 2004, 126, 4786-4787.
R. Daneshfar and J.S. Klassen “Arrhenius Activation Parameters for the Loss of Neutral Nucleobases from Deprotonated Oligonucleotide Anions in the Gas Phase” J. Am. Soc. Mass Spectrom. 2004, 15, 55-64.

2003 Publications

W. Wang, E.N. Kitova and J.S. Klassen “Bioactive Recognition Sites May Not Be Energetically Preferred in Protein-Carbohydrate Complexes in the Gas Phase” J. Am. Chem. Soc. 2003, 125, 13630-13631.
W. Wang, E.N. Kitova and J.S. Klassen “Influence of Solution and Gas Phase Processes on Protein-Carbohydrate Binding Affinities Determined by Nanoelectrospray Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry” Anal. Chem. 2003, 75, 4945-4955.
W. Wang, E.N. Kitova and J.S. Klassen “Determination of Protein-Oligosaccharide Binding by Nanoelectrospray Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry” Methods in Enzymology 2003, 362, Editors: Y.C. Lee and R.T. Lee, Elsevier, Amsterdam, 376-397.

2002 Publications

E.N. Kitova, W. Wang, D.R. Bundle and J.S. Klassen “Retention of Bioactive Ligand Conformation in a Gaseous Protein-Trisaccharide Complex” J. Am. Chem. Soc. 2002, 124, 13980-13981.
N. Felitsyn, E.N. Kitova and J.S. Klassen “Thermal Decomposition of the Protein Homodimer Ecotin in the Gas Phase” J. Am. Soc. Mass Spectrom. 2002, 13, 1432-1442.
E.N. Kitova, D.R. Bundle and J.S. Klassen “Evidence for the Preservation of Specific Intermolecular Interactions in Gaseous Protein-Oligosaccharide Complexes” J. Am. Chem. Soc. 2002, 124, 9340-9341.
E.N. Kitova, D.R. Bundle and J.S. Klassen “Thermal Dissociation of Protein-Oligosaccharide Complexes in the Gas Phase. Mapping the Intrinsic Intermolecular Interactions” J. Am. Chem. Soc. 2002, 124, 5902-5913.

2001 Publications

N. Felitsyn, E.N. Kitova and J.S. Klassen “Thermal Decomposition of a Gaseous Multiprotein Complex Studied by Blackbody Infrared Radiative Dissociation. Investigating the Origin of the Asymmetric Dissociation Behaviour” Anal. Chem. 2001, 73, 4647-4661.
E.N. Kitova, P. Kitov, D.R. Bundle and J.S. Klassen “The Observation of Multivalent Complexes of the Shiga-Like Toxin with Globotriaoside and the Determination of their Stoichiometry by Nanoelectrospray Fourier-Transform Ion Cyclotron Resonance Mass Spectrometry” Glycobiology 2001, 11, 605-611.